Pulsed Field Gel Electrophoresis (PFGE)

PFGE involves the enzymatic digestion of whole microbial chromosomes using appropriate restriction enzyme. Microbial typing is achieved by separating digested fragments in a specific electrophoresis system under conditions such as constant alternating changes in the direction of the electric field and appropriate pulse times.

PFGE is recognized as a "golden standard" for strain typing of common pathogenic microorganisms, with good reproducibility and high resolution. PFGE plays an important role in the traceability investigation of foodborne pathogens and the identification of strain typing, which is recommended by USP and CHP. The picture below shows the PFGE equipment.