Microbial Identification

CICC is the unique national industrial culture collection in China, with a history of nearly 60 years of strain classification and identification. CICC has advanced bioscience instruments and a team of professional personnel engaged in strain classification and identification. CICC has passed ISO 9001 Quality Management System Certification in 2012, ISO 17025 National Laboratory Accreditation in 2016 and CMA Qualification Accreditation for Inspection and Testing Organizations in 2018, respectively. CICC undertakes the commissioning and identification of industrial microbial strains in China, and the technical services of microbial identification and evaluation have obtained the "State Administration for Industry and Commerce (SCIC) business license". CICC provides microbial microbial identification services covering a wide range of microorganisms, such as bacteria (actinomycetes), yeasts and filamentous fungi, for customers about scientific research institutions, colleges and universities and industries. The microbial identification methods include molecular biological identification (16S rDNA, 26S rDNA, ITS rDNA and house-keeping genes), morphological observation, VITEK automatic microbial identification, API physiological and biochemical identification, BIOLOG carbon source automatic analysis and identification, MALDI-TOF MS rapid identification, whole genome sequencing analysis, etc.

Identification items

Item number	Detection Item	workdays	Description
CICC-Bseq03	16S rDNA	7	Bacteria
CICC-Yseq03	26S rDNA D1/D2 region	7	Yeast
CICC-Yseq05	5.8S rDNA ITS region	7	Filamentous fungi
CICC-Bseq07	16S rDNA	7	Bacteria
CICC-Yseq07	26S rDNA D1/D2region	7	Yeast
CICC- Fseq10	5.8S rDNA ITS region	7	Filamentous fungi
CICC-Identi	Species level identification	10	Bacteria、Yeast
CICC-Identi	Species level identification	20	Actinomycetes, Molds
CICC-BHGen01	gyrA gene	5	Genus Bacillus
CICC-BHGen02	gyrBgene	5	Genus Pseudomonas, Bacillus, Enterobacter, Klebsiella
CICC-BHGen03	pheSgene	5	Genus Lactobacillus, Enterococcus
CICC-BHGen04	recNgene	5	Genus Leuconostoc, Streptococcus
CICC-BHGen05	rpoBgene	5	Genus Staphylococcus
CICC-BHGen06	trpBgene	5	Genus Streptomyces
CICC-BHGen07	atpDgene	5	Genus Bifidobacterium
CICC-FHGen01	β-tubulingene	5	Genus Aspergillus, Penicillium, Mucor
CICC-FHGen02	calmodulingene	5	Genus Aspergillus, Penicillium
CICC-FHGen03	EF1-αgene	5	Genus Mucor, Rhizopus, Trichoderma
CICC-FHGen04	Actingene	5	Genus Mucor, Rhizopus
CICC-BIOLOG	BLOLOG	7	Automatic identification system based on carbon source metabolism
CICC-API	API	10	Bacteria、Yeast
CICC-BIOM	Physiology and biochemistry	10	Bacteria、Yeast
CICC-BIOM	Physiology and biochemistry	20	Actinomycetes
CICC-BIOMN	Physiology and biochemistry (novel species、distinct species)	20	For potential novel species, references requirements for novel species test
CICC-SEM	Scanning electron microscope	20	Observation of morphological features (morphology, surface)
CICC-TEM1	Transmission electron microscope (Negative staining)	20	Observation of morphological features (accessory structures)
CICC-TEM2	Transmission electron microscope (Ultrathin section)	20	Observation of morphological features (internal)
CICC-GC1	（G+C）mol%	10	Tm value method
CICC-GC2	（G+C）mol%	15	HPLC method
CICC-DDH	DNA-DNA hybridization	20	DNA homology determination of strains genome
CICC-Lipid	Fatty acid	15	Chemical analysis of bacteria and actinomycetes
CICC-Quin	Ubiquinone/Methylnaphthoquinone	15
CICC-PLipid	Polar lipid	15
CICC-CWS	Cell wall component (sugar)	15
CICC-CWA	Cell wall component (amino acid)	15
CICC-RAPD	RAPD	15	Strain typing, for strain traceability, property rights disputes, etc
CICC-SSCP	SSCP	15
CICC-RepP	Rep-PCR	15
CICC-EricP	Eric-PCR	15
CICC-BoxP	Box-PCR	15
CICC-ARDRATyp	ARDRA Restriction Enzyme digestion	10
CICC-RiboTyp	Ribotyping	15
CICC-PFGE	PFGE	25
CICC-Isolation	Strain isolation and purification	15	Isolation and purification of strains from mixed samples
CICC-Count	Colony counting of specific bacteria	15	Lactobacillus, Bifidobacterium, phosphorus bacteria, nitrifying bacteria, denitrifying bacteria
CICC-Gstab	Genetic stability	30	Genetic stability test for production strains
CICC-Enzyme	Enzymatic activity assay	10	Protease/amylase/pectinase/phytase/cellulase/saccharase/catalase/dehydrogenase/urease, etc
CICC-Bsus	Bacterial Antimicrobial Susceptibility	15	Paper diffusion method/Broth dilution method/Agar dilution method /Etest
CICC-Ysus	Yeast Antimicrobial Susceptibility	15	Broth dilution method/Agar dilution method /Etest
CICC-Fsus	Fungi Antimicrobial Susceptibility	15	Broth dilution method/Agar dilution method
CICC-BIOLOGECO	BIOLOG ECO plate analysis	15	Microbial community analysis is used for non-culture microbial community analysis of mixed samples in environment, water quality, traditional brewing industry, etc
CICC-DGGE	DGGE sample preparation and testing	30
	DGGE electrophoresis
	DGGE cutting, purification, cloning and sequencing
	DGGE result analysis
CICC-16SLib	Sample preparation and testing	30
	16S rDNA clone library construction
	Clone screening and sequencing (200 clones)
	Result analysis
CICC-MAnaly	Product microbiological analysis (culture method)	20	Microbial fermentation product control
CICC-Contamin	Contaminated bacteria analysis (culture method)	20
CICC-Phage	Phage detection (Negative)	5
CICC-Phage	Phage detection (Positive)	15
CICC-FBroth	Fermentation broth preparation (30L)	15
CICC-PTRCRCICC	Standard preparation and testing	20	Microbial quantitative analysis is used for relative quantitative/absolute quantitative analysis of foodborne pathogenic bacteria, clinical, industrial and other microbial strains
	Sample preparation and testing
	Relative quantification (Taqman probe method /SYBR Green dye method)
	Absolute quantification (Taqman probe method /SYBR Green dye method)
	Result analysis
CICC-Clo	Clostridium botulinum detection	7	SNT 2525-2010 PCR method for detection of Clostridium botulinum in food
CICC-Virulence	Strain virulence	60	Suitable for the safety evaluation of microbial strains
CICC-antigene	Antibiotic resistance gene detection	15	Suitable for the safety evaluation of microbial strains
CICC-antivirus	Antiviral ability evaluation	30	Suitable for the functional evaluation of microbial strains
CICC-MetaC	Metabolite content determination	10	Acid, alcohol, ketone, etc., suitable for the functional evaluation of microbial strains
CICC-Fmor	Morphological identification	20	Filamentous fungi
CICC-LAMP	Loop-mediated Isothermal Amplification	10	Microbial rapid diagnosis
CICC-metag	Metagenomic analysis	50	Miseq PE sequencing
CICC-wgen	Whole genome analysis	60	illumina Hisea2000
CICC-genoextra	Nucleic acid extraction	10	Efficient extraction of microbial genome /mRNA/ total RNA
CICC-expB	Expression and purification of bacterial protein expression system	35	Construction of efficient expression system of proteins without modification, optimization of initiation intensity, plasmid copy number and expression conditions.
CICC-expY	Expression and purification of yeast protein expression system	35	The construction of protein eukaryotic expression system includes the optimization of activation intensity and expression conditions
CICC-antipre	Antigen design and preparation	60	Sequence optimization of large molecular antigens (above 10kD) and structure design of small molecular antigens. Antigen preparation services include the preparation of macromolecules, small molecules, bacteria, fungi and other bacterial antigens.
CICC-antitag	Antibody labeling	20	Biotin labeling/enzyme labeling/fluorescein labeling
CICC-antimT	Qualitative and semi-quantitative analysis of antigenic substances	10	Target antigen: protein/small molecule/bacteria/virus
CICC-proT	Protein qualitative and quantitative analysis	15	/
CICC-TOF03	MALDI-TOF MS rapid identification	3	Bacteria, yeast, filamentous fungi
CICC-TOF01	MALDI-TOF MS rapid identification	1	Bacteria, yeast, filamentous fungi