Whole Genome Sequencing, WGS

Whole genome sequencing (WGS) is the process of determining the sequence of an organism’s genome. WGS can comprehensively and accurately analyze the base composition of an organism’s genome and reveal the complexity and diversity of the genome. Since the first commercial automatic sequencer appeared in 1987, sequencing technology has developed rapidly and sequencing costs continue to decline. Microbial WGS has become a widely used technology in molecular biology laboratories. Compared with traditional microbial identification methods, WGS has incomparable advantages and has become a cutting-edge technology with great development potential in current biological research. WGS has been included in newly issued regulations and guidelines in the fields of food, medicine, feed and other fields, providing important methods for the systematic classification, strain typing, function mining and safety evaluation of microorganisms. CICC has established a set of standardized operating procedures including genomic DNA extraction, library preparation, DNA sequencing, bioinformatics analysis, etc., that can achieve rapid and accurate determination of the entire genome sequence of microorganisms.

Schematic diagram of microbial Whole Genome Sequencing（Su et al., 2019）