Metagenomic sequencing
Environmental microorganisms are the most widely distributed, diverse and abundant biological groups in nature. Excavation and utilization of new environmental microbial resources is one of the important contents of current environmental microbial research. At present, the research on microorganisms in the environment is mainly based on pure culture. However, the latter often has a relatively long cycle and a relatively large workload. In recent years, high-throughput sequencing technology has developed rapidly. Metagenomics technology can avoid traditional microbial isolation and culture methods, and use all DNA in environmental samples as the research object. Total DNA can be directly extracted from environmental samples, and libraries can be constructed and sequenced. Information can be obtained such as the composition, abundance and metabolic pathways of microorganisms in the sample through bioinformatics analysis. Metagenomic sequencing analysis can clarify the types and relative abundance of microorganisms in samples, analyze differentiated metabolic pathways, and help explore the relationship among microorganisms, environment, and host. Metagenomic analysis has been widely used in food, cosmetics, feed, home appliances, clinical and other fields, providing solutions for microbial structure analysis in traditional fermented foods, probiotic product composition, contaminating bacteria traceability, intestinal microecology research, etc.